熒光/化學光成像儀


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【Cool Imager?】- 高靈敏生物發光與熒光數字成像儀 (IMGR002, IMGR003)

一、產品背景常規的生物圖像與生物信號用肉眼觀察靈敏度低或根本無法進行肉眼觀察。常常需要采用酶顯色或放射性同位素放大后進行觀察。近年興起的Chemiluminescence(化學發光)因其極高靈敏度與優越的線性關系得到普遍應用。用光學照相機照像或膠片顯影法顯示結果則需要暗室以及膠片,照相紙,沖洗液等耗材。圖像的定量分析與后期處理也較困難。相比之下,通過數字相機的數字成像技術則有許多優點。國內市場上出售的電泳圖像成像系統因靈敏度低而不能用于顯示化學自發光反應 (Chemiluminescence)以及熒光示蹤。結合現代生物學,光學,半導體及計算機技術,我公司開發了高靈敏度的數字成像系統(Cool Imager)。 Cool Imager為多用途數字成像及圖像分析工作站,主要用于酶催化的化 學自發光反應與熒光圖譜。由于對攝像系統采用了深低溫冷卻(-45oC)技術,靈敏度較傳統的酶底物顯色法提高約2000倍。

完全成像系統包括所有硬件、并已經安裝系統與分析軟件,開箱后即可使用。高度集成的系統已經包括微機,不需要另配計算機。系統已經優化。系統啟動與關閉迅速。采像單元可每分鐘采積并累加近2000幅圖。最高累加圖幅達200,000幅圖,采像時間可達25分鐘。一般取圖5,000幅左右即可在計算機屏幕清晰顯示蛋白印跡圖或分子雜交圖。由于采圖數是任意可變的,Cool Imager可擴展用于攝取DNA/RNA凝膠圖,考馬氏亮蘭及銀染的蛋白凝膠圖,以及蛋白熒光圖譜。

本工作臺可連接顯微攝像頭攝取彩色顯微鏡圖像。
  

二、技術指標

系統冷卻達-45oC,可以使微弱的發光成像。曝光25分鐘、累集10,000幅圖沒有明顯雜色背景。
  

三、技術參數(CoolImager II)

 

 

 

 

 

 

 

 

 

 

MAIN UNIT

Complete System

Cool Imager™ is an all-in-one unit and comes with all the hardware including the monitor, keyboard, mouse, pre-installed operating system and analytical software. After you receive the units, plug in and use; that is it. No custom hardware and software installation are required.

High Integration

The unit is integrated with the computer-board with an embedded CPU, the control board and operating panel, optical devises, a frame grabber, lights, filters and a mini darkroom chamber. This specific design increases efficiency and stability of the workstation.

Low-noise

Technology

Low-noise technology is applied to all the electronic components in the unit to prevent the interruption of electro-noise and reduce vibration, which makes the design of highly integrated system possible and enhances the stability of the unit.

Sensitivity

Detects as little as ~0.5 fg (10-17g) of HRP with Lightgen™ substrates and completely eliminates the use of X-ray film for chemiluminescent imaging.

Imaging Capacity

Cool imager can integrate ~30 frames per second and collect ~50,000 frames in ~30min, and its sensitivity is better than autoradiography using radioisotope p32 or S35.

Darkroom

The mini darkroom is light-proofed by a design with multi-staged, magnetic sealing strips, which permit the door to open and close easily.

Self-contained

Unit

There is no external camera or lens outside of the unit. All electronic and optical components are enclosed inside the unit, which provides a moisture-proof, dust-proof environment and extend the service life of the electronic and optical components.

Power

Operation

Workstation uses a flat control panel to turn on and off the operation program and also controls image capturing, light source, power lens and camera functions.

 

 

 

SOFT-Ware

Operation

Pre-installed Windows® (Microsoft) or Linux®  (Redhat) operation program

Imaging & Analyzing Software

With the software, ViaCapture, capturing an image is achieved simply by entering the number of integration. 1D gel, 2D dot-blot, bacterial colony, Western blots, DNA/RNA hybridization, EMSA and microplates can be imaged and saved by Cool Imager™. The captured images can be opened and edited with ViaAnalysis software. ViaAnalysis can invert, correct slanted images, adjust image contrast and brightness, perform image quantitation and more. All images can be copied, pasted or printed.

Other programs

Office, Image editor, PDF, Zip and Antivirus programs are pre-installed.

C C D

The ultra-cooled, C-Mount camera with 2-stage Peltier cooling mechanism, cools temperature down to -45oC and reduces the background noise down to 17 e- rms. The cooled camera has an 8 bit, ½ inch CCD installed with a pixel size of 6.4um X 6.4um. The resolution can reach up to 1642H X 1024V.

LENS

F=1:1.0, 8~48mm optical lens with power zoom, power focus and iris.

LIGHTS

A set of white reflective lights and 3 sets of colored reflective lights (Cold Light) are included. Transmitting blue, ultra-violet or white light boxes are sold separately at an additional cost.

FILTERS

A tunable filter wheel which can hold 4 sets of filters. A brown filter is included for imaging DNA/RNA gels stained with Ethidium bromide or SYBR Green. Other filters are options sold separately at an additional cost.

注; 攝像機的冷卻溫度與儀器靈敏度有關。溫度越低,雜色越少,靈敏度越高,價格高。 VersaDoc未配置變焦鏡頭,沒有圖像放大功能,不能用于基因芯片圖。 CoolImager配專門技術用于我公司推出的EMSA系列成套試劑。其他公司的儀器不能顯示結果。Cool Imager本身具有所有控制單元的軟,硬件。只需外接顯示器即可使用。

 

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